Web Reference: Pairwise sequence alignment is the process of aligning two sequences to each other by optimizing the similarity score between them. In this tutorial, you will learn how to perform pairwise sequence alignment in Biopython using the modern PairwiseAligner interface. You will see how to do global and local alignment, change scoring rules, inspect results, and work with both nucleotide and protein sequences. Learn how to perform global and local pairwise sequence alignments in Biopython using the pairwise2 module. Step-by-step guide with code examples for bioinformatics analysis.
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Last Updated: April 7, 2026
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